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SFD-10HT

SFD-10HT

Eliminates DNA Fragments <10 kb

The First Magnetic bead-based reagent for efficient elimination of short DNA Fragments 

Short Fragment Depletor-10 High Throughput (SFD-10HT) reagent is a bead-based reagent used for size selection and purification of high molecular weight DNA in long read sequencing. It can significantly improve the mean read length by near elimination of DNA fragments below 10 kb. This kit is suitable for amplicons, sheared/fragmented DNA and genomic DNA. SFD-10HT working concentration range is 10-150 ng/µL. 

Performance Data
Comparative Data
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Available Options

SKUDescriptionPriceQuantity
SF-80005SFD-10HT (5 mL)$ 375.00
SF-80050SFD-10HT (50 mL)$ 2,500.00
  • High Molecular Weight DNA: purification and concentration 
  • Sheared/fragmented DNA: elimination of fragments <10 kb 
  • Library cleanup and rapid size selection >10 kb 
  • Enhancement of DNA mean read length by elimination of short fragments (<10 kb) prior to library preparation 
  • During SFD-10HT size selection and short fragment depletion, DNA is purified, which helps eliminate contaminants that may be carried over from the extraction process.
  • Concentrates dilute HMW DNA samples 
  • Tolerant to a wide range of DNA input range (10-150 ng/µL) 
  • Applicable in a wide variety of sample types: HMW DNA, fragmented DNA, sheared DNA, and PCR amplicons/libraries 
  • Bead-based – no centrifugation  
  • Efficient scale-up and flexibility – amenable to automation 
  • Reproducible results 
  • High recovery efficiency (50-90%) depending on quality and concentration of input DNA 
  • Saves time – protocol less than 65 minutes 

The Short Fragment Depletor-10 High Throughput (SFD-10HT) reagent is designed for the cleanup and size selection of sheared or fragmented DNA in third-generation sequencing. SFD-10HT uses bead-based size selection to drastically eliminate short DNA fragments below 10 kb. Short DNA fragments (<10 kb) are progressively eliminated and very large fragments are enriched to improve the mean read length. SFD-10HT is an ideal solution for enhancement of reads’ quality in long-read sequencing workflows.

Features Specifications
Main functions  Progressively eliminates DNA fragments <10 kb and rapidly selects long DNA libraries 
Starting material (Sample matrix)  Sheared/fragmented DNA, amplicons/libraries, HMW DNA 
Starting amount  10-150 ng/µL of DNA 
DNA recovery  50-90% depending on input DNA quality and concentration 
Process method  Manual or automation 
Instrument  Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request 
Purification method  Magnetic bead-based technology 
DNA purity  DNA eluted is ready for downstream processes such as library preparation for third generation sequencing  
Elution volume  10 ng/µL or above

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