HighPrep HMW DNA Kit

The HighPrep High Molecular Weight DNA Kit is a magnetic bead-based kit for high molecular weight DNA isolation from whole blood, bone marrow, saliva, buccal cells, cultured cells, tissues, and bacteria.

Step-by-Step Protocol for High Molecular Weight DNA Isolation Using the HighPrep HMW DNA Kit
Watch this step-by-step video to learn how to use the HighPrep HMW DNA Kit. This kit guides you through four steps: Lysis, DNA Binding, Bead Wash, and DNA Elution, ensuring high purity and yield (50-300+ kb).

Performance Data

Comparative Data

Available Options

SKU	Description	Price	Quantity
HPHMW-D96	HighPrep HMW DNA Kit (96 preps)	$ 864.00	HighPrep HMW DNA Kit quantity
HPHMW-D96x4	HighPrep HMW DNA Kit (984 preps)	$ 2,688.00	HighPrep HMW DNA Kit quantity

HMW DNA isolation is suitable for molecular applications such as:

Long read sequencing
Next generation sequencing
Microarray
PCR amplification and genotyping
Cloning
Restriction enzyme digestion

Isolation of high-quality, high molecular weight DNA 50-300+ kb
HMW DNA is suitable for all third-generation sequencing platforms including Nanopore and PacBio SMRT sequencing
Achieve high yields of DNA from a wide range of sample matrices
Efficient scale-up and flexibility
Amenable to automation and has easy to use protocols (Kingfisher™ scripts are ready)
Comes with RNase to minimize the co-purification of RNA

The HighPrep High Molecular Weight DNA Kit is specifi cally designed for isolation of high molecular weight DNA (HMW DNA) in the size range of 50–300+ kb. The kit extracts HMW DNA from biological samples such as whole blood, bone marrow, saliva, buccal cells, cultured cells, tissues, and bacteria. The HighPrep High Molecular Weight DNA Kit utilizes magnetic bead-based technology in combination with chaotropic agents to gently isolate HMW DNA and remove inhibitors. The HMW DNA purifi ed with this kit is particularly suitable for analysis on long read sequencing genomic platforms including PacBio RSII/Sequel/Sequel II and Oxford Nanopore. DNA purifi ed using the HighPrep High Molecular Weight DNA Kit is of good quality and purity. The kit includes RNase A for the removal of RNA. The exact size of extracted DNA varies depending on sample matrix, the quality of the starting material, and processing conditions.

Protocol

Features	Specifications
Starting Material (Diverse Sample Types)	200 μL of blood, bone marrow, and saliva, up to 10 mg of tissue, up to 1 x 10^9 Cultured cells, bacterial pellet with OD600=1 or less, 200 μL buccal swab cells in medium
Process Method	Supports manual and automated processing
Instrument	Adaptable to most nucleic acid purification instruments, but a ready-made script for Kingfi sher™ Flex is available upon request.
Purification Method	Magnetic bead-based technology
Elution Volume	30-100 μL
Size Range	50-300+ kb
Purity	A260/A280 and A260/A230 of >1.7
Yield	Depends on the quality and amount of sample. 10 mg of tissue yields 10 μg of DNA or higher. 200 μL of human whole blood yields 3-7 μg of DNA.
Automated Extraction on Kingfi sher™ Flex	1 hr. 20 mins
Manual Extraction	2 hrs.
Downstream Applications	Long read sequencing, Next generation sequencing, Real-time quantitative PCR, Reverse transcriptase PCR, PCR, Microarray, Genotyping, Restriction enzyme digestion

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HighPrep HMW DNA Kit

Available Options

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